Chemo-immune cell therapy by intratumoral injection of adoptive NK cells with capecitabine in gastric cancer xenograft model

Introduction: Gastric cancer is one of the most commonly known malignancies and fifth cancer-related death globally. Whereas natural killer (NK) cells play a critical role in tumor elimination; therefore, adoptive NK cell therapy has become promising approach cytotherapy. Hence, this study investigated chemo-immune MKN-45 derived xenograft gastric model. Methods: Three groups animals have received following treatments separately: activated cells, capecitabine, combination capecitabine was considered as control group. Morphometric properties samples were evaluated at end study. infiltration by immunohistochemistry (IHC) hCD56. Mitotic count treatment response assessed hematoxylin eosin (H&E) staining. The proliferation ratio to apoptosis determined IHC assessment Ki67 caspase 3. Results: results indicated that could effectively decrease mitotic pathology assessment, but not completely eradicated. In with metronomic chemotherapy (MC) demonstrated significant difference morphometric compared also line data. Conclusion: Although vivo, obtained findings lesser potency than MC despite ex vivo activation. order enhance effectiveness, suppressive features microenvironment inhibitory immune checkpoints blockade should be considered.